Animal Modeling Professional Services Animal models of human diseases occupy a very important place in medical science research. The biological Animal center of Mai Rui actually after more than 20 years of development, accumulated a wealth of animal culture and model building experience, in the animal model construction methods to explore a unique experience, the average animal model to build the mean success rate of more than 98%, the problem model (open chest/craniotomy, etc.) to build an average success rate of more than 75%, The formation of high efficient and high quality animal model construction method database can successfully build hundreds of disease animal models. Establishment of Rat Mcao model Mcao is the abbreviation of Middle cerebral artery occlusion, Chinese meaning is the cerebral artery embolism, this topic uses the line bolt method, the line bolt method has the effect affirmation which does not have the craniotomy, may accurately control the ischemia and the reperfusion time merit. The middle cerebral artery embolism model does not plug the cord into the middle of the brain artery, but through the internal carotid artery into the skull and into the anterior cerebral artery, blocking the blood supply from the anterior cerebral artery from the embolism side and blocking the intracranial segment of the internal carotid artery that receives the blood supply from the artery. 1, the experiment steps: 1, the mice were weighed 2, the mice were anesthetized-3, the preparation of sterile special cord and experimental surgical instruments-4, with medical tape fixed mice-5, shave the mouse neck hair-6, neck with alcohol cotton sponge disinfection-7, neck median incision-8, homemade hook exposure vision-9, separation of the common carotid artery-10, Isolated artery (outside neck/neck)-11, ligation of the common carotid artery-12, ligation of the external carotid artery-13, Standby line -14, "V" type incision-15, insert the bolt-16, fixed cord-17, layer by stitch-18, circular verification model-19, fist verification model. 2, Note: 1, when separating the neck muscle, the two sides small muscle called the bubble gland (many people mistakenly think is the thyroid gland), avoids the injury to cause the haemorrhage. 2, to see the carotid sheath, we should pay attention to the careful separation of the vagus nerve, if the injury may affect its breathing and death. 3, the separation of blood vessels, as far as possible to peel the blood vessels clean, so that in the eye scissors will not mistakenly cut the outer membrane, and to cut a diagonal incision. 4, fasten the line around in the CCA far heart of the thin lines when attention to grasp the strength, must be tied, or bleeding more, but not too tight, or plug line difficult. 5, The wire bolt is a little bit inserted, instead of clamping the middle part of the wire bolt into the immediately, until a slight resistance to stop the insertion line. Center-prepared animal model (part)