Primary cell culture 1. Primary culture of lymphocytes (main source of lymphocytes: spleen, peripheral blood) Steps: 1, extract peripheral blood-2, add lymphocyte separation Liquid-3, add blood-4, after centrifugal blood stratification-5, aspiration mononuclear cell-pbmc cell 2. Primary culture of rat cerebral cortex neurons Steps: 1, Neonatal rat culture-2, alcohol soaked mice-3, peel mouse brain tissue-4, select cerebral Cortex-5, peel blood vessels-6, wash-7, shear digestion-8,200 mesh stainless steel mesh filtration 3. Cell identification The cells were cultured for 1 days/2 days/3 days under different periods of photo, and Immunofluorescence was identified.